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. 2014 Feb;13(2):267–278. doi: 10.1128/EC.00268-13

FIG 4.

FIG 4

SSU2 deletion raises nitrate uptake and nitrate induction. (A) Nitrate uptake. Cells grown in synthetic medium plus 5 mM ammonium were resuspended in the same medium at an OD660 of 2 to 3 but without a nitrogen source and incubated for 90 min. Nitrate uptake was determined as extracellular nitrate depletion for 90 min. Assays were triggered with 0.5 mM nitrate. Data ± SE from three independent experiments are shown. (B) Nitrate induction. Strains bearing YNR1-lacZ grown in synthetic medium plus 5 mM ammonium were resuspended in nitrogen-free medium for 90 min. YNR1-lacZ induction in response to 1 mM nitrate was determined as β-galactosidase activity and expressed as nmol o-nitrophenol · min−1 (mU) · mg−1 of protein. Data ± SE from three independent experiments are shown.