TABLE 1.
Assay name | Primer or probe | Primer or probe sequence (5′–3′)a | Target organism | Reference |
---|---|---|---|---|
Salmon | Forward primer | GGTTTCCGCAGCTGGG | Salmon (Oncorhynchus keta) testes | 56 |
Reverse primer | CCGAGCCGTCCTGGTCTA | |||
Probe | FAM-AGTCGCAGGCGGCCACCGT-TAMRA | |||
Entero1 | Forward primer | AGAAATTCCAAACGAACTTG | Enterococcus | 57 |
Reverse primer | CAGTGCTCTACCTCCATCATT | |||
Probe | FAM-TGGTTCTCTCCGAAATAGCTTTAGGGCTA-TAMRA | |||
EPA-EC 23S | Forward primer | GGTAGAGCACTGTTTTGGCA | E. coli | 58 |
Reverse primer | TGTCTCCCGTGATAACTTTCTC | |||
Probe | FAM-TCATCCCGACTTACCAACCCG-TAMRA | |||
Genbac | Forward primer | GGGGTTCTGAGAGGAAGGT | Bacteroidales | 28 |
Reverse primer | CCGTCATCCTTCACGCTACT | |||
Probe | VIC-CAATATTCCTCACTGCTGCCTCCCGTA-Iowa Black | |||
CowM3 | Forward primer | CCTCTAATGGAAAATGGATGGTATCT | Cattle-associated microbial population | 29 |
Reverse primer | CCATACTTCGCCTGCTAATACCTT | |||
Probe | FAM-TTATGCATTGAGCATCGAGGCC-TAMRA | |||
Rum-2-bac | Forward primer | ACAGCCCGCGATTGATACTGGTAA | Ruminant-associated microbial population | 30 |
Reverse primer | CAATCGGAGTTCTTCGTGAT | |||
Probe | FAM-ATGAGGTGGATGGAATTCGTGGTGT- TAMRA |
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.