Skip to main content
. 2014 Jan;80(1):43–53. doi: 10.1128/AEM.02223-13

FIG 4.

FIG 4

Confirmation of tyrosine-to-DOPA conversion. (A) Tyrosinase was added to E. coli XL10-Gold and recombinant E. coli cells harboring pOW19F-PerPA1, pOW19F-PerPA2, pOW19F-PerPA3, and pOW19F-PerPA4. Aggregation was observed only for tyrosinase-treated recombinant E. coli harboring pOW19F-PerPA3(PerPA3-1DOPA) and pOW19F-PerPA4 (PerPA4-2DOPA). ∗, tyrosinase treatment. (B to F) TEM analysis of XL10-Gold cells (B) and XL10-Gold cells harboring pOW19-PerPA1 (C), pOW19F-PerPA2 (D), pOW19F-PerpA3 (E), and pOW19F-PerPA4 (F) after incubation with tyrosinase and gold solution (HAuCl4). Gold nanoparticle formation was only observed in the PerPA3-DOPA and PerPA4-DOPA E. coli cells.