TABLE 1.
Primers used in PCR experiments
| Primer sequence (5′–3′)a | Gene to be amplified | Template |
|---|---|---|
| GAATTCATGAAAAAGTTAACAGTGGCG | Truncated OmpW_forward | E. coli W3110 gDNA |
| TCTAGAGCCATGATCGTTAAATCCATT | Truncated OmpW (aa 139)_reverse | E. coli W3110 gDNA |
| TCTAGATGCACCGCCCAGCTTATAAT | Truncated OmpW (aa 191)_reverse | E. coli W3110 gDNA |
| TCTAGAATGGGTTACGTGACAACGAAGG | Perhydrolase_forward | P. aeruginosa PAO1 gDNA |
| AAGCTTTCAGCCGCGGATGAAGGCC | Perhydrolase_reverse | P. aeruginosa PAO1 gDNA |
| AAGCTTTTAATGGTGATGATGGTGATGGCCGCGGATGAAGGC | Perhydrolase + 6-His_reverse | P. aeruginosa PAO1 gDNA |
| AAGCTTTTAATAATGGTGATGATGGTGATG | Perhydrolase + 6-His + 1Tyr_reverse | Perhydrolase + 6-His PCR product |
| AAGCTTTTAATAATAATGGTGATGATGGTGATG | Perhydrolase + 6-His + 2Tyr_reverse | Perhydrolase + 6-His PCR product |
a
Restriction enzyme sites are indicated in boldface.