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The clo3403 and clo3404 mutants show impaired growth at 12°C. Growth curves of WT and clo3403s, clo3403a, clo3404s, and clo3404a strains at 12°C and 30°C. Volumes of 350 μl of diluted ONCs (1:100 diluted into fresh TPGY broth) were incubated at 12°C or 30°C in the Bioscreen C microbiology reader in an anaerobic workstation. OD600 values were measured automatically at 15-min intervals. Error bars represent standard deviations of three biological replicates.
