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The clo3403s mutant does not form flagella. Electron microscopy images of WT and clo3403s strains. The C. botulinum ONCs were diluted (1:100) into 50 ml of fresh TPGY broth in duplicate and incubated at 12°C or 30°C. Samples for transmission electron microscopy were taken during mid-exponential growth phase at 12°C and 30°C.
