FIG 7.

Average Raman spectra of dormant and germinated B. subtilis spores after various HP treatment times. (A) Average Raman spectra of 150-MPa HP-treated wild-type B. subtilis spores that released their DPA after treatment for various times, as described in Materials and Methods. (B) Average Raman spectra of 150-MPa HP-treated spores that retained their DPA after treatment for various times. Fifty individual spores were randomly analyzed by LTRS, and the spectra of the spores that released their DPA were averaged. In order to obtain the average spectra of the spores that retained their DPA, the spectra of 15 more spores that retained their DPA in each sample were measured and averaged.