TABLE 1.
Germination parameters of Bacillus spores treated with various HPsa
| Strain, treatment | Tlag (min) | Trelease (min) | ΔTrelease (min) | ΔTlys (min) | Ilag | Irelease | No. of spores examined (% germinated) |
|---|---|---|---|---|---|---|---|
| PS533, 140 MPa | 13.2 ± 12.5 | 15.8 ± 14.6 | 2.6 ± 0.9 | 11.8 ± 6.3 | 0.76 ± 0.10 | 0.20 ± 0.06 | 372 (95) |
| PS533, heat activated, 140 MPa | 12.7 ± 10.4 | 15.2 ± 10.8 | 2.5 ± 0.6 | 9.8 ± 4.2 | 0.75 ± 0.07 | 0.17 ± 0.06 | 359 (96) |
| PS533, 50 MPa | 28.5 ± 16.0 | 31.3 ± 16.0 | 2.8 ± 1.0 | 12.5 ± 6.8 | 0.78 ± 0.11 | 0.19 ± 0.06 | 182 (61) |
| FB111, 140 MPa | 12.5 ± 8.1 | 34.1 ± 11.3 | 21.5 ± 9.0 | NA | 0.82 ± 0.09 | 0.12 ± 0.07 | 359 (96) |
| B. cereus, 140 MPa | 0.35 ± 0.06 | 0.71 ± 0.11 | 0.36 ± 0.10 | 9.0 ± 3.3 | 0.99 ± 0.01 | 0.25 ± 0.06 | 139 (98) |
| PS533, heat activated, 10 mM l-alanine | 15.5 ± 15.9 | 18.2 ± 16.2 | 2.7 ± 0.9 | 13.1 ± 6.8 | 0.87 ± 0.10 | 0.24 ± 0.08 | 562 (91) |
a
Spores of B. subtilis strain PS533 (wild type) with or without heat activation, B. subtilis strain FB111 (cwlJ), or B. cereus without heat activation were treated with various HPs, and germination was followed for 60 min, as described in Materials and Methods. Data from >100 individual spores were used to calculate the germination parameters shown. Data represent mean values and standard deviations. NA, not available.