TABLE 2.
Germination parameters of B. subtilis spores treated with 140 MPa of HP and then incubated at 1 MPaa
| HP treatment | Tlag (min) | Trelease (min) | ΔTrelease (min) | ΔTlys (min) | Ilag | Irelease | No. of spores examined (% germinated) |
|---|---|---|---|---|---|---|---|
| 140 MPa, 10 min | 12.3 ± 8.5 | 15.4 ± 8.5 | 3.0 ± 0.9 | 13.0 ± 5.6 | 0.83 ± 0.06 | 0.19 ± 0.06 | 189 (97) |
| 140 MPa, 2 min | 12.5 ± 8.9 | 15.6 ± 8.8 | 3.1 ± 0.8 | 12.5 ± 7.3 | 0.86 ± 0.07 | 0.21 ± 0.07 | 271 (95) |
| 140 MPa, 30 s | 12.8 ± 11.1 | 15.4 ± 11.0 | 2.8 ± 0.9 | 10.7 ± 5.0 | 0.81 ± 0.07 | 0.19 ± 0.05 | 528 (93) |
| 140 MPa, 10 s | 14.5 ± 12.0 | 17.5 ± 12.0 | 3.0 ± 0.8 | 14.3 ± 5.8 | 0.77 ± 0.09 | 0.20 ± 0.07 | 558 (95) |
| 140 MPa, constant | 13.2 ± 12.5 | 15.8 ± 14.6 | 2.6 ± 0.9 | 11.8 ± 6.3 | 0.76 ± 0.10 | 0.20 ± 0.06 | 372 (95) |
a
Wild-type B. subtilis spores were incubated at 37°C and 140 MPa for short periods and then incubated at 37°C and 1 MPa for a total of ∼60 min, as described in Materials and Methods. Data from >100 individual spores were used to calculate the germination parameters shown. Data represent mean values and standard deviations.