TABLE 3.
Germination parameters of B. subtilis spores treated with 150 MPa of HP and then incubated at 0.1 MPaa
| HP treatment | Tlag (min) | Trelease (min) | ΔTrelease (min) | ΔTlys (min) | Ilag | Irelease | No. of spores examined (% germinated) | No. of germinated spores analyzed |
|---|---|---|---|---|---|---|---|---|
| 150 MPa, constant | 4.5 ± 3.9 | 7.7 ± 4.2 | 3.0 ± 1.1 | 9.6 ± 4.2 | 0.88 ± 0.08 | 0.26 ± 0.06 | 275 (95) | 170 |
| 150 MPa, 5 min | 4.3 ± 2.1 | 7.2 ± 2.6 | 2.9 ± 1.6 | 9.0 ± 4.7 | 0.88 ± 0.05 | 0.27 ± 0.08 | 459 (90) | 200 |
| 150 MPa, 2 min | 3.4 ± 1.8 | 5.9 ± 2.3 | 2.5 ± 0.8 | 12.0 ± 3.8 | 0.90 ± 0.06 | 0.28 ± 0.07 | 306 (75) | 113 |
| 150 MPa, 1 min | 2.9 ± 1.7 | 5.2 ± 2.0 | 2.3 ± 0.7 | 6.7 ± 1.7 | 0.90 ± 0.11 | 0.23 ± 0.11 | 180 (30) | 55 |
| 150 MPa, 10 s | 2.8 ± 2.5 | 4.8 ± 3.2 | 2.0 ± 1.6 | 8.4 ± 6.0 | 0.91 ± 0.06 | 0.20 ± 0.09 | 315 (7.6) | 19 |
a
Wild-type B. subtilis spores were incubated at 37°C with 150 MPa for short periods and then incubated at 37°C and ambient pressure (0.1 MPa) for a total of ∼60 min, as described in Materials and Methods. Data represent mean values and standard deviations.