TABLE 1.

Preliminary LoD determination by the U.S. group for four PCR assays with six inhibitor-resistant PCR reagents in varying total reaction concentrations of DPBS, soil, and whole blood

Assay and reagent	LoDa with the indicated % of:
	1× DPBS		Whole blood				Soil
	25.0	5.0	2.50	0.50	0.25	0.05	0.25	0.05	0.025	0.005
BAPA
Phire	10 pg	10 fg	ND	20 fg	50 fg	10 fg	ND	10 fg	10 fg	10 fg
Phusion	50 fg	2 pg	50 fg	2 pg	50 fg	2 fg	50 fg	2 fg	10 fg	2 fg
KAPA	50 fg	10 fg	50 fg	10 fg	100 fg	10 fg	1 pg	200 fg	10 fg	20 fg
Fast Virus	100 fg	20 fg	ND	2 pg	1 pg	200 fg	1 pg	200 fg	100 fg	200 fg
Phire plus STRboost	10 fg	2 pg	ND	ND	10 pg	2 pg	1 pg	2 pg	100 fg	2 pg
Phire plus Ampdirect	50 fg	200 fg	ND	10 fg	ND	200 fg	ND	200 fg	100 fg	200 fg
Omp2A
Phire	50 fg	200 fg	1 pg	10 fg	100 fg	200 fg	10 pg	10 fg	100 fg	200 fg
Phusion	10 fg	200 fg	ND	ND	ND	200 fg	10 fg	2 fg	50 fg	2 pg
KAPA	10 fg	200 fg	1 pg	200 fg	100 fg	200 fg	100 fg	200 fg	50 fg	2 pg
Fast Virus	1 pg	200 fg	10 pg	2 pg	100 fg	200 fg	50 fg	20 fg	10 fg	200 fg
Phire plus STRboost	1 pg	200 fg	10 pg	2 pg	50 fg	200 fg	50 fg	200 fg	50 fg	200 fg
Phire plus Ampdirect	50 fg	200 fg	100 fg	200 fg	10 pg	200 fg	ND	10 fg	10 fg	200 fg
VEEV NSP
Phire	10 fg	10 fg	ND	200 fg	10 fg	20 fg	ND	10 fg	10 fg	2 fg
Phusion	10 fg	2 fg	ND	200 fg	ND	2 fg	1 pg	2 fg	10 fg	10 fg
KAPA	10 fg	2 fg	10 pg	ND	50 fg	2 fg	10 fg	2 fg	10 fg	2 fg
Fast Virus	50 fg	20 fg	ND	ND	10 pg	2 pg	10 fg	10 fg	10 fg	20 fg
Phire plus STRboost	50 fg	10 fg	50 fg	100 fg	50 fg	50 fg	10 fg	10 fg	50 fg	20 fg
Phire plus Ampdirect	10 fg	10 fg	ND	ND	10 pg	200 fg	ND	2 pg	10 fg	10 fg
OP HA
Phire	10 pg	200 fg	ND	2 pg	ND	20 fg	ND	ND	1 pg	2 pg
Phusion	100 fg	200 fg	ND	ND	1 pg	20 fg	1 pg	200 fg	10 fg	20 fg
KAPA	100 fg	200 fg	1 pg	200 fg	50 fg	20 fg	50 fg	20 fg	10 fg	200 fg
Fast Virus	1 pg	2 pg	10 pg	2 pg	1 pg	200 fg	50 fg	20 fg	50 fg	200 fg
Phire plus STRboost	100 fg	200 fg	ND	2 pg	50 fg	10 fg	10 fg	20 fg	10 fg	10 fg
Phire plus Ampdirect	100 fg	200 fg	ND	200 fg	ND	200 fg	ND	2 pg	50 fg	200 fg

^aThe lowest concentration at which both replicates of duplicate samples yielded positive results. ND, not detected.