TABLE 3.
Determination by the U.S. group of LoDs for four PCRs with the KAPA and Fast Virus reagents within whole blood
| Assay | KAPA reagent |
Fast Virus reagent |
||
|---|---|---|---|---|
| LoD (% matrixa) | Cqb (CVc) | LoD (% matrix) | Cq (CV) | |
| BAPA | 100 fg (2.5) | 33.51 (3.01) | 2 pg (0.05) | 30.61 (0.82) |
| Omp2A | 200 fgd (0.5) | 33.93 (16.31) | 200 fg (0.05) | 34.51 (2.53) |
| VEEV NSP | 10 pg (0.25) | 26.75 (1.12) | 2 pg (0.05) | 30.56 (3.72) |
| OP HA | 1 pg (2.5) | 35.18 (6.28) | 200 fg (0.05) | 36.36 (2.23) |
a
% matrix, percentage of blood in the reaction mixture.
b
PCR cycle number at which fluorescence was first detected in 45-cycle PCR.
c
CV, coefficient of variation, calculated as [(standard deviation of the mean/mean) × 100] and expressed as a percentage.
d
Twenty-nine of 30 replicates were positive.