TABLE 1.
Primers and probes used in RT-qPCR
| Target | Purpose | Concn of primers/concn of probe (nM) | Reference |
|---|---|---|---|
| PSTVd | Detection of PSTVd | 900/200 | 21 |
| Confirmation of doubtful results with universal pospiviroid amplicon | 300/100 | 22 | |
| PVY | Detection of PVY with PVYuni amplicon | 300/150 | 23 |
| PepMV | Detection of PepMV with Eur-cp or Ch2-US2-cp amplicons | 900/200 | 24 |
| Detection of PepMV in the exptl hydroponic system | 200/400 | 25 | |
| COX mRNA (COX) | Control for extraction of plant tissue samples | 900/200 | Adapted from reference 26 |
| Luciferase RNA (LUC) | Control for extraction of water samples | 1,000/500 | 27 |