TABLE 4.
Presence of PVYNTN in source plants, nutrient solution, and bait plantsa
| Expt | No. of daysb | Presence of PVYNTN |
|||||
|---|---|---|---|---|---|---|---|
| Source of inoculum |
Nutrient solution |
Bait plants |
|||||
| Leaves | Roots | RT-qPCR | No. of positive plant/no. of all test plantsc | Roots | Upper parts | ||
| PepMV+PVY-tomato | 0–30 | + (15) | + (22) | + (36–39) | 0/12 | − | − |
| 31–60 | NT | + (22–25) | + (38)/−d | 0/12 | + (35–38) | − | |
| 61–90 | NT | + (20) | − | 0/4 | + (35) | − | |
| 91–120 | + (14) | + (23) | + (36)/−d | 0/4 | − | + (39)/−d | |
| 134 | NT | + (21–23) | + (34) | 0/2 | + (34–37) | + (36)/−d | |
| NC | 0–134 | − | − | − | − | − | − |
| PVY-potato | 0–30 | + (12–20) | + (18–29) | + (33–39) | 0/12 | + (36–38)/−d | − |
| 31–60 | NT | NT | + (30–35) | 0/12 | + (27–37)/−d | + (36)/−d | |
| 61–90 | NT | NT | + (28–36) | 0/12 | + (26–37) | + (33–39)/−d | |
| 91–120 | NT | NT | + (33–38) | 0/3 | + (24–36) | − | |
| 131 | + (13–18) | + (19–22) | + (39) | NT | + (34–38) | + (35–39)/−d | |
| NC | 0–131 | − | − | − | − | − | − |
a
RT-qPCR and test plants were used for the detection. In parentheses are the ranges of mean Cq values (as the means of three replicates) for positive samples. +, positive; −, negative. NT, not tested; NC, control plants, negative isolation, and RT-qPCR controls.
b
Days after initiation of irrigation with infested nutrient solution.
c
Test plants were inoculated with nutrient solution and tested 4 weeks later.
d
Different samples were tested; some of these were positive, the others negative.