TABLE 6.
Presence of PSTVd in plants grown in substrate treated with PSTVd-infested water and in secondary plants germinated from tubers
| Infected water (Cq) | Bait plants (no.)b | No. of positive plants/no. of all secondary plantsa |
|
|---|---|---|---|
| 10 wks | 16 wks | ||
| PSTVd (24 ± 1) | Potato tubers, cv. Hermes (4) | 0/1 | 0/4 |
| Potato tubers, cv. Donald (4) | 0/2 | 3 (18–31)/6 | |
| Potato tubers, cv. Nicola (4) | 0/3 | 2 (17–31)/5 | |
| Negative control | Potato tubers, cv. Hermes (4) | 0/0 | 0/2 |
| Potato tubers, cv. Donald (4) | 0/3 | 0/3 | |
| Potato tubers, cv. Nicola (4) | 0/1 | 0/1 | |
a
RT-qPCR was used for the detection. In parentheses are the ranges of mean Cq values (as the means of three replicates) for positive samples.
b
Infection was not confirmed in the green parts of bait plants.