TABLE 1.
Strains used in this study
| Strain | Descriptiona | Source or reference |
|---|---|---|
| PY79 | Prototrophic laboratory strain | R. Losick, Harvard University, USA |
| NCIB 3610 | Prototrophic isolate, reference sequence NC_000964.3 (Bacillus subtilis strain 168) | Bacillus Genetic Stock Center, Ohio State University, USA |
| BEST40401 | RM125 (hsdR hsdM leu arg) pLS20cat | 14 |
| SB505 | 3610 tasA::spc | 15 |
| DS1796 | PY79 motAB::tet | D. Kearns, Indiana University, USA |
| GP397 | 3610 yueB::tet | J. E. Gonzalez-Pastor laboratory |
| GP399 | 3610 yukBA::tet | J. E. Gonzalez-Pastor laboratory |
| DS313 | PY79 sigD::tet | 16 |
| AKR04 | PY79 pLS20neo | 8 |
| TCR06 | PY79 thrC::mls | 8 |
| TCR07 | 3610 pLS20cat | This study |
| TCR08 | 3610 tasA::spc pLS20cat | This study |
| TCR09 | PY79 motAB::tet pLS20neo | This study |
| TCR10 | 3610 yueB::tet pLS20cat | This study |
| TCR11 | 3610 yukBA::tet pLS20cat | This study |
| TCR12 | PY79 sigD::tet pLS20neo | This study |
| TCR13 | PY79 pLS20neo amyE::Pxyl-yueB-yfp | This study |
| TCR15 | PY79 pLS20neo yukBA::tet amyE::Pxyl-yueB-yfp | This study |
| LH1 | PY79 thrC::Physpank::cds7 pLS20neo | This study |
| LH2 | PY79 thrC::Physpank::cds8 pLS20neo | This study |
| WG 8 | PY79 thrC::Physpank::rapLS20 | This study |
a
cat, chloramphenicol; spc, spectinomycin; tet, tetracycline; neo, neomycin-kanamycin; mls, lincomycin-erythromycin.