FIG 7.
Equilibrium dissociation constants between CYP154C3 and the substrates. (A) Shift of the spin state of the heme (type I spectral shift) induced by the binding between CYP154C3 and Δ4-androstene-3,17-dione (final concentrations are 0, 0.195, 0.391, 0.781, 1.56, 3.13, 6.25, 12.5, and 25.0 μM). The shift accompanied by the increasing substrate concentrations is indicated by arrows. (B) Apparent Kd values of CYP154C3 and each substrate. Values indicate the means from three independent experiments with standard deviations.