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A pC3-cured derivative of B. cenocepacia K56-2 lacked pathogenicity in a zebrafish embryo model of infection. Embryos were microinjected with wt K56-2, K56-2/pBBR::AtoxA, and K56-2 ΔpC/pBBR::AtoxA with, on average, 225, 246, and 253 CFU, respectively, in the blood circulation and incubated at 28°C. Mortality, determined by the absence of a heartbeat, is presented as Kaplan-Meier curves. Significance was determined using the log-rank (Mantel-Cox) test and was found to be <0.001.
