FIG 5.

H111 is more resistant to osmotic stress than its pC3-null derivative. (A) Bacteria were grown overnight in LB medium and then subcultured into LB supplemented with additional NaCl to a total concentration of 2 M. Viability was measured before inoculation into high-salt LB broth and after 2.5, 5, and 24 h of incubation at 37°C with shaking in this medium. (B) Morphological differences between H111 and H111Δc3 colonies plated from bacteria grown for 2.5 h growth in high-salt LB. The photographs are to the same scale.