FIG 2.
P(3HB) synthesis by E. coli JM109 harboring the high-copy-number plasmid pGEM-phaRCYB-4AB (dashed line) and the low-copy-number plasmid pBBR1-phaRCYB-4AB (solid line). (A) Cell dry weight (open circles) and P(3HB) content (filled triangles); (B) ethanol (EtOH) concentration in the broth; (C) synthase activity (act.) and Western blot analysis of PhaCYB-4 after 24 h of cultivation; (D) Mn and Mw; (E) Mw/Mn. Cells were cultivated in 500-ml shake flasks containing 100 ml LB medium plus glucose (20 g/liter) at 37°C. Each experiment was carried out in triplicate.