TABLE 1.
Bacterial strains and plasmids used in the study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| E. coli JM109 | recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) e14− (mcrA) supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+ lacIq lacZΔM15] | TaKaRa Bio |
| E. coli BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm | Novagen |
| Plasmids | ||
| pBBR1MCS-2 | Broad-host-range vector, Kmr | 13 |
| pBBR1-phaRCYB-4AB | pBBR1MCS-2 derivative; phaRe promoter, phaRCYB-4 from B. cereus YB-4, and phaABRe from R. eutropha | 12 |
| pJRD215 | Broad-host-range vector, Kmr | 14 |
| pJRD-phaCDaAB | pJRD215 derivative; phaRe promoter, phaCDa from D. acidovorans DS-17, and phaABRe from R. eutropha H16 | This study |
| pGEM″ABex | pGEM-T derivative, phaRe promoter, phaABRe from R. eutropha H16, Apr | 15 |
| pGEM-phaCDaAB | pGEM″ABex derivative; phaCDa from D. acidovorans DS-17 (identical to pGEM″ABexCaC) | 16 |
| pGEM-phaCReAB | pGEM″ABex derivative; phaCRe from R. etutropha H16 | 17 |
| pGEM- phaRCYB-4AB | pGEM″ABex derivative; phaRCYB-4 from B. cereus YB-4 | 7 |
| pGEM-phaRCBmAB | pGEM″ABex derivative; phaRCBm from B. megaterium NBRC15308T | 7 |
| pGEM-phaRYB-4AB | pGEM″ABex derivative; phaRYB-4 from B. cereus YB-4 | 7 |
| pGEM-phaCYB-4AB | pGEM″ABex derivative; phaCYB-4 from B. cereus YB-4 | 7 |
| pGEM-phaRCYB-4 | pGEM-T derivative; phaRe promoter, phaRCYB-4 from B. cereus YB-4 | This study |
| pET15b | T7 promoter, His tag fusion protein | Novagen |
| pET15b-phaRYB-4 | pET15b derivative; phaRYB-4 from B. cereus YB-4 | This study |
| pET15b-phaCYB-4 | pET15b derivative; phaCYB-4 from B. cereus YB-4 | This study |