TABLE 4.
In vitro incubation of the P(3HB)–PhaRCYB-4 complex in phosphate buffer with or without ethanola
| Sample | Incubation with additive | Incubation time (h) | Mol wt of P(3HB) |
||
|---|---|---|---|---|---|
| Mn (103) | Mw (103) | Mw/Mn | |||
| A | No incubation | 0 | 180 ± 9 | 365 ± 30 | 2.0 |
| B | No additive | 24 | 193 ± 10 | 355 ± 16 | 1.8 |
| C | Ethanol | 24 | 17 ± 3 | 23 ± 7 | 1.4 |
a
P(3HB) polymerization using purified PhaRCYB-4 was performed for 3 min at 30°C, and the reaction mixture was divided into three equal samples, A, B, and C, by volume. Sample A represented the initial condition prior to incubation. Samples B and C were centrifuged, and the precipitates were incubated for 24 h at 30°C in fresh phosphate buffer (pH 7.0) without and with ethanol (2.6 g/liter), respectively. Results are the averages ± standard deviations from three separate experiments.