FIG 2.

Abolition of the FA beta-oxidation pathway increases lipid accumulation in A. gossypii. (A) Simplified diagram of FA biosynthesis and degradation in A. gossypii. ACL activity (gray) is not present in A. gossypii. The dashed arrows indicate a multistep pathway. (B) Total FA quantification of A. gossypii mutant strains lacking beta-oxidation genes. Analyses were carried out with cultures grown in 8% glucose (glc) or 1% glucose plus 2% oleic acid for 3 days. The results are the means of two independent experiments performed in duplicate. The error bars represent the standard deviations. C.D.W., cell dry weight.