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. 2014 Feb;80(4):1237–1244. doi: 10.1128/AEM.03560-13

FIG 3.

FIG 3

Heterologous expression of the Y. lipolytica ACL genes in A. gossypii. (A) Domain organizations of ACL-encoding genes in different species. S. pombe, Schizosaccharomyces pombe; A. thaliana, Arabidopsis thaliana; R. oryzae, Rhizopus oryzae; U. maydis, Ustilago maydis. (B) Relative transcription levels of the ACL2 and ACL1 genes in our A. gossypii mutant strains. Transcription levels were normalized using the A. gossypii ACT1 gene as a reference. Relative quantitative analyses were performed using LightCycler 480 software. The results are the means of two independent experiments performed in duplicate and are expressed as a ratio of the cDNA abundances of the target genes with respect to the ACT1 mRNA levels. (C) Acetyl-CoA quantification in A. gossypii strains. The results are the means of two independent experiments performed in duplicate. (D) Total FA quantification in A. gossypii strains. Analyses were carried out with cultures grown in 8% glucose for 3 days. The results are the means of two independent experiments performed in duplicate. The error bars represent the standard deviations.