FIG 4.

Lipid accumulation in the engineered A. gossypii strains. (A) Lipid quantification in A. gossypii strains at different time points of cultures grown in 1% glucose plus 2% oleic acid at 0 to ∼50 h (trophic phase) and 50 to 168 h (productive phase). The results are the means of two independent experiments performed in duplicate. The error bars represent the standard deviations. (B) Micrographs of the WT and pox1Δ strains of A. gossypii grown in 1% glucose plus 2% oleic acid for 7 days. Lipid bodies were stained with Bodipy and visualized under fluorescence microscopy.