FIG 4.
Lcl secreted in the extracellular milieu is not sufficient to induce the aggregation of Lp02 Δlpg2644. (A) Anti-Lcl immunoblots of the supernatant (SN) and cell fraction (CF) from sedimentation assays with Lp02, Lp02 Δlpg2644, and mixed suspensions. (B and C) Fluorescence microscopy analysis of labeled autoaggregates after 5-h static incubations with Lp02/pGFP and Lp02 Δlpg2644/pRFP (B) and Lp02/pRFP and Lp02 Δlpg2644/pGFP (C) mixed suspensions in deionized water with 500 μM MgCl2. (D and E) Relative quantifications of Lp02 and Lp02 Δlpg2644 in mixed sedimentation assays from panels B and C, respectively. Scale bars, 10 μm. *, statistically significant differences (P ≤ 0.001 by the two-tailed Student t test).