FIG 5.

GLBRCY87 displays greater tolerance to ferulic acid than GLBRCY73 during xylose consumption. In panel A, the cell viabilities of GLBRCY73 or GLBRCY87 grown in culture tubes at 30°C with the indicated media were measured. At the indicated times, the number of live or dead cells were counted flow cytometry, and the average percent cell viability and standard deviations from 3 independent biological replicates were calculated. Asterisks indicate statistically significant differences (Student t test: *, P < 0.03; **, P < 0.04). In panels B to D, representative growth profiles of xylose uptake by engineered and evolved yeast strains in YPX media containing 2.4 g/liter pCA or FA for strain GLBRCY73 (B) and strain GLBRCY87 (C) and comparison of xylose consumption by the 2 strains (D) are shown.