TABLE 1.
Primers used in this study
| Primer | Sequence | Usea |
|---|---|---|
| Hypo_1 | GCTGAAGGAAGAGCCTGGCCTCCTCGG | TTC1624 and TTC1623 |
| PGP_1 | GGAGCTTCCAGCATGCGTATGAAACGGGTG | |
| PGP_3 | CCGAGCGCTTCGCCCGCGTGCAGCGCCTCC | TTC1623 and pilF (TTC1622) |
| PilF_5′_rev2 | CGGGAAGGCCGAGCTCCGGGTAGTGTTTGG | |
| PilF_3′_for1 | GGGACTCCGAGACGGCCAAGATCGCCACC | pilF (TTC1622) and pilT1 (TTC1621) |
| PilT_1 | GGTCCTCAATGGTGACGATGTGGCAGGCCTTGCGTTC | |
| PilT_2 | CCATAGACCGCATCGTGGACGTCTTCCC | pilT1 (TTC1621) and TTC1620 |
| Gluta_1 | GCTGAAGGAAGAGCCTGGCCTCCTCGG |
a
Use as a bridge between the indicated two genes.