FIG 5.

Neighbor-joining tree showing the phylogenetic relationship of the type strain (in bold) of the proposed species “Ferrovum myxofaciens” (belonging to the proposed family “Ferrovaceae” and order “Ferrovales”) to 16S rRNA gene sequences of some closely related clones and orders within the Betaproteobacteria. 16S rRNA gene sequences within the proposed order “Ferrovales” form at least six monophyletic groups. Numbers at the nodes represent bootstrap values out of 1,000 replicates, and GenBank accession numbers for sequences are given in parentheses. Bootstrap values in bold indicate branches which were not supported by the other analysis methods used. The scale bar represents 2% sequence divergence. The tree was rooted with the Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans type strains (not shown).