TABLE 4.
Summary of the number of sequences analyzed, observed richness (OTUs), estimated OTU richness (ACE and Chao1), evenness, diversity indices (Shannon and Simpson), and estimated sample coverage of pyrosequences
| Group | No. of reads | No. of OTUs | ACE | Chao1b | Shannon | Evennessa | Simpsonc | Coveraged |
|---|---|---|---|---|---|---|---|---|
| LF | 358 | 5 | 5 | 5 (5, 5) | 0.70 | 0.44 | 1.55 | 100.0 |
| HF | 414 | 6 | 6 | 6 (6, 6) | 0.37 | 0.21 | 1.17 | 100.0 |
| HRFI | 379 | 6 | 6 | 6 (6, 6) | 0.58 | 0.32 | 1.35 | 100.0 |
| LRFI | 393 | 6 | 7 | 6 (6, 6) | 0.53 | 0.30 | 1.32 | 99.8 |
a
Calculation based on the formula by Pielou (61) (J′ = H′/H′max), where H′ is the Shannon diversity index and H′max is the logarithm of the number of species.
b
Values in parentheses are 95% confidence intervals.
c
Calculated as Simpson's reciprocal index (1/D).
d
Calculation based on the formula by Good (34) [C = 1 − (n/N)], where n is the number of unique clones and N is the total number of clones.