FIG 4.

Summary of total free fatty acid titer and in vitro assay data, normalized to the respective values for C. taiwanensis FabG, which produced 11.1 mg/liter total free fatty acids. Titers are from separate E. coli DH1ΔfadE strains overexpressing ′tesA and a fabG variant under anaerobic conditions. The control strain overexpressed a codon-optimized sequence of the native E. coli fabG. Total free fatty acid titer (black bars), NADH specific activity (white bars), and ratio of NADH:NADPH specific activities (hatched bars) are shown. Bar heights represent average titers from 3 replicates, and error bars represent 1 standard deviation.