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. 2014 Jan;196(1):80–89. doi: 10.1128/JB.00988-13

FIG 2.

FIG 2

“Ca. Kuenenia stuttgartiensis” cells display S-layers as observed by TEM after multiple types of sample preparation. (A) “Ca. Kuenenia stuttgartiensis” cells are covered by a hexagonal S-layer, as observed after freeze-etching. (B) The FFT power spectrum of a part of the S-layer seen in panel A reflects the regular pattern of the S-layer. The p6 symmetry and center-to-center spacing of 20 nm for the S-layer of “Ca. Kuenenia stuttgartiensis” are reflected by the FFT power spectrum. (C) A freeze fracture through the S-layer gives an inside view into the “Ca. Kuenenia stuttgartiensis” cell underneath. (D) The S-layer (indicated by the arrow) forms a zigzag layer on top of the cytoplasmic membrane in “Ca. Kuenenia stuttgartiensis” cells that were cryofixed; freeze-substituted in acetone containing 2% osmium tetroxide, 0.2% uranyl acetate, and 1% water; Epon embedded; and thin sectioned. Scale bars, 200 nm.