FIG 5.
Analysis of crude extract and enriched S-layers of “Ca. Kuenenia stuttgartiensis” by SDS-PAGE. (A) SDS-PAGE gel stained with Coomassie G shows all proteins present in the “Ca. Kuenenia stuttgartiensis” crude extract. (B) SDS-PAGE gel stained with Coomassie G shows the proteins present after S-layer enrichment. (C) SDS-PAGE gel stained with periodic acid-Schiff's reagent shows glycoproteins present after S-layer enrichment. Arrows indicate protein bands in which Kustd1514 was detected via LC-MS/MS analysis.