FIG 7.

(A to C) Immunogold localization of the antiserum directed at Kustd1514 localizes the protein to the S-layer surrounding the cells in rehydrated “Ca. Kuenenia stuttgartiensis” cryosections. In panel A, for clarification, white circles indicate gold labels that are localized inside the cytoplasmic membrane (so not directly on the S-layer). Considering the length of the antibody-protein A-gold complex (25 nm), these gold labels most likely also correspond to S-layer labeling (except for the one in the anammoxosome). (D) Negative control incubated with preimmune serum instead of antiserum. Scale bars, 500 nm.