TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| S. mutans | ||
| UA159 | Wild type, serotype c | 22 |
| IBS16 | UA159 derivative, ΔSMU.746, Kmr | This study |
| IBSL28 | UA159 derivative, ΔSMU.747, Kmr | This study |
| IBSL23 | UA159 derivative, ΔSMU.745, Kmr | This study |
| IBSL32 | UA159 derivative, ΔSMU.746-SMU.747, Kmr | This study |
| IBSL32ΔK | IBSL32 derivative, Kms | This study |
| 3S3F | UA159 with ISS1 inserted in codon 146 of SMU.747 | This study |
| 7A5G | UA159 with ISS1 inserted in codon 172 of SMU.747 | This study |
| 61B11 | UA159 with ISS1 inserted in codon 177 of SMU.747 | This study |
| E. coli | ||
| EC100 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 galU galKλ− rpsL (strR) nupG | Epicentre |
| Plasmids | ||
| pGEM-T EZ | Commercial TA cloning vector, Apr | Promega |
| pUC18 | Commercial cloning vector, Apr | Invitrogen |
| pIB107 | GusA promoter probe vector, Kmr | 34 |
| pIB184-Km | S. mutans expression shuttle vector, Kmr | 33 |
| pIBC62 | pGEM-T EZ derivative with a 2.27-kb fragment containing SMU.747 and SMU.746, Apr | This study |
| pIBC77 | pGEM-T EZ derivative with a 1.34-kb fragment containing SMU.747, Apr | This study |
| pIBL09 | pUC18 derivative with a 1.73-kb fragment containing SMU.745, Apr | This study |
| pIBL17 | pIBL09 derivative for deletion of SMU.745, Apr Kmr | This study |
| pIBL20 | pIBC62 derivative for deletion of SMU.746, Apr Kmr | This study |
| pIBL24 | pIBC77 derivative for deletion of SMU.747, Apr Kmr | This study |
| pIBL28 | pIBC62 derivative for deletion of SMU.746, Apr Kmr | This study |
| pIBL30 | pIB107 with 373-bp SMU.747 promoter fragment, Kmr | This study |
| pIBL31 | pIB107 with 163-bp SMU.745 promoter fragment, Kmr | This study |
| pIBL36 | pIB184K expressing SMU.746-SMU.747, Apr Kmr | This study |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Emr, erythromycin resistance.