FIG 2.

Sequence alignment and structural analysis of mPDF and sPDF. (A) Pairwise alignment of nucleotide-derived amino acid sequences of mPDF and sPDF. Cysteines are highlighted in boxes. Notations used: st, S. Typhimurium; mt, M. tuberculosis; double dots, conserved substitutions; single dots, semiconserved substitutions; stars, identical residues. (B) Positioning of different cysteines in protein structures. A superposition of the mPDF structure (pink; PDB ID 3E3U) with the E. coli PDF (EcPDF) structure (gray; PDB ID 3K6L) is shown. Important residues are labeled for EcPDF, and the corresponding residues in mPDF are indicated within parentheses. The metal ion from EcPDF is represented as a sphere (red).