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UV-visible spectra of NBD-Cl-modified sPDF (A) and C130M-V63C (B) proteins in response to H2O2 exposure. NBD-Cl (20-fold molar excess of protein) was added to dialyzed protein following incubation with or without a 100-fold molar excess of H2O2. Unbound NBD-Cl was removed by washing, and the labeling was detected by a spectral scan (260 to 600 nm) as described in Materials and Methods.
