E. coli strains |
|
|
DH5α |
Δ(argF-lac)169 ϕ80dlacZ58(M15) ΔphoA8 glnV44 deoR481 gyrA96 recA1 endA1 hsdR17
|
Lab stock |
EC1000 |
Emr; MC1000 derivative carrying single copy of pWV01repA in glgB
|
Lab stock |
E. faecalis strains |
|
|
FA2-2 |
Rifampin/fusidic acid-resistant mutant derived from plasmid-free strain JH2 |
30 |
FAZL1 |
ΔfabI of E. faecalis FA2-2 |
18 |
BHK260 |
Spontaneous RBS mutant S1 of FAZL1, C-12 to G |
This work |
BHK261 |
Spontaneous RBS mutant S2 of FAZL1, C-12 deletion |
This work |
BHK262 |
Spontaneous RBS mutant S3 of FAZL1, A-9 to G |
This work |
BHK263 |
Spontaneous RBS mutant S4 of FAZL1, T-19 to G |
This work |
FAZL6 |
FA2-2 fabK (disruption) |
This work |
FAZL7 |
FAZL1 fabK (disruption) |
This work |
FAZL8 |
S1 fabK (disruption) |
This work |
FAZL9 |
S2 fabK (disruption) |
This work |
FAZL10 |
S3 fabK (disruption) |
This work |
FAZL11 |
S4 fabK (disruption) |
This work |
Plasmids |
|
|
pTRKL2 |
Ermr, low-copy-no. E. coli/Lactococcus shuttle vector |
42 |
pCR2.1 |
Ampr Kanr, TOPO TA cloning vector |
Invitrogen |
pZL52 |
Ampr Kanr, pCR2.1 carrying E. faecalis FAZL1 fabK plus 275 bp upstream and 288 bp downstream |
This work |
pZL53 |
Ampr Kanr, pCR2.1 carrying E. faecalis mutant S1 (strain BHK260) fabK plus 275 bp upstream and 288 bp downstream |
This work |
pZL54 |
Ampr Kanr, pCR2.1 carrying E. faecalis mutant S2 (strain BHK261) fabK plus 275 bp upstream and 288 bp downstream |
This work |
pZL55 |
Ampr Kanr, pCR2.1 carrying E. faecalis mutant S3 (strain BHK262) fabK plus 275 bp upstream and 288 bp downstream |
This work |
pZL56 |
Ampr Kanr, pCR2.1 carrying E. faecalis mutant S4 (strain BHK263) fabK plus 275 bp upstream and 288 bp downstream |
This work |
pZL157 |
Ermr, EcoRV and BamHI-digested fabK fragment of pZL52 inserted into same sites of pTRKL2 |
This work |
pZL158 |
Ermr, EcoRV- and BamHI-digested fabK fragment of pZL53 inserted into same sites of pTRKL2, C-12 to G |
This work |
pZL159 |
Ermr, EcoRV- and BamHI-digested fab fragment of pZL54 inserted into same sites of pTRKL2, C-12 deletion |
This work |
pZL160 |
Ermr, EcoRV- and BamHI-digested fabK fragment of pZL55 inserted into same sites of pTRKL2, A-9 to G |
This work |
pZL161 |
Ermr, EcoRV- and BamHI-digested fabK fragment of pZL56 inserted into same sites of pTRKL2, T-19 to G |
This work |
pBHK368 |
Ermr, fabK A-9T of pZL157 |
This work |
pBHK369 |
Ermr, fabK A-9C of pZL157 |
This work |
pBHK370 |
Ermr, fabK C-12A of pZL157 |
This work |
pBHK371 |
Ermr, fabK C-12T of pZL157 |
This work |
pBHK372 |
Ermr, fabK T-19A of pZL157 |
This work |
pBHK373 |
Ermr, fabK T-19C of pZL157 |
This work |
pMC1871 |
Tetr, promoterless lacZ reporter plasmid |
32 |
pBHK322 |
Ermr, lacZ from pMC1871 cloned into BamHI site of pTRKL2, p(lacZ) |
This work |
pBHK323 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, p(WT) |
This work |
pBHK394 |
Ermr, pTRKL2 fabK (−275 to +35)::lacZ
|
This work |
pBHK324 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, C-12 to G, p(S1) |
This work |
pBHK325 |
Ermr, pTRKL2 P32::fabK (−136 to + 35)::lacZ, C-12 deletion, p(S2) |
This work |
pBHK326 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, A-9 to G, p(S3) |
This work |
pBHK327 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, T-19 to G, p(S4) |
This work |
pBHK334 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, A-9 to T |
This work |
pBHK335 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, A-9 to C |
This work |
pBHK336 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, C-12 to A |
This work |
pBHK337 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, C-12 to T |
This work |
pBHK338 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, T-19 to A |
This work |
pBHK339 |
Ermr, pTRKL2 P32::fabK (−136 to +35)::lacZ, T-19 to C |
This work |