TABLE 2.
Oligonucleotide primers used in this study
| Oligonucleotide | Sequencea |
|---|---|
| fabKup275 | ATTAGACGCGGACAGCTTAG |
| fabKdo288 | TACGACATCAGGCGTTAGTC |
| P32-f | ACTCGCCTGCAGAGATTAATAG TTTTAGCTA |
| fabKfusion-r | ATACTTGTCGACTTGATTCCAAGCAACTCACA |
| P32FabK-f | CTAGGTAGGTAAAAAAATATTACATGAAAATAGGGGGCT |
| P32FabK-r | AGCCCCCTATTTTCATGTAATATTTTTTTACCTACCTAG |
| fabKup275-PstI | AATATCTGCAGATTAGACGCGGACAGCTTAG |
| fabK-random-f | TAGTGGATCCATTAGACGCGGACAGCTTAGA |
| fabK-random-r | ATATCTGCAGTTCACTTAGCCCCAACGCTGAT |
| Ef16S-f | CATCATGCCCCTTATGACCTGG |
| Ef16S-r | CCTGCAATCCGAACTGAGAGA |
| lacZ-f | ATGAACGGTCTGGTCTTTGC |
| lacZ-r | ATCGCTATGACGGAACAGGT |
| fabK-RT-f | GGCAATCCAGCCAAATACAT |
| fabK-RT-r | ATGTCCACCAGCTTCCATTC |
| c-12a-f | GAAGTGTACTTATCTTAGAACTAAAGGAAGTATAAAATCAATGAATCAACAGTTATG |
| c-12a-r | CATAACTGTTGATTCATTGATTTTATACTTCCTTTAGTTCTAAGATAAGTACACTTC |
| c-12t-f | GAAGTGTACTTATCTTAGAACTAAAGGATGTATAAAATCAATGAATCAACAGTTATG |
| c-12t-r | CATAACTGTTGATTCATTGATTTTATACATCCTTTAGTTCTAAGATAAGTACACTTC |
| a-9c-f | GTACTTATCTTAGAACTAAAGGACGTCTAAAATCAATGAATCAACAGTTATGT |
| a-9c-r | ACATAACTGTTGATTCATTGATTTTAGACGTCCTTTAGTTCTAAGATAAGTAC |
| a-9t-f | GTACTTATCTTAGAACTAAAGGACGTTTAAAATCAATGAATCAACAGTTATGT |
| a-9t-r | ACATAACTGTTGATTCATTGATTTTAAACGTCCTTTAGTTCTAAGATAAGTAC |
| t-19a-f | CTTGAATGAAGTGTACTTATCTTAGAACAAAAGGACGTATAAAATCAATGAATC |
| t-19a-r | GATTCATTGATTTTATACGTCCTTTTGTTCTAAGATAAGTACACTTCATTCAAG |
| t-19c-f | CTTGAATGAAGTGTACTTATCTTAGAACCAAAGGACGTATAAAATCAATGAATC |
| t-19c-f | GATTCATTGATTTTATACGTCCTTTGGTTCTAAGATAAGTACACTTCATTCAAG |
| fabK inside For | ATGCGAGCTCTGAAGAACAGGTTCCTGTCG |
| fabK inside Rev | ATCGCTGCAGCTGCATCTAAATCACGTGCC |
| pBVGh For (P1) | GTACCTACGTAGGATCGATC |
| pBVGh Rev (P2) | GCTTCCAAGGAGCTAAAGAG |
| fabK check Up (P3) | ATTAGACGCGGACAGCTTAG |
| fabK check Down (P4) | TACGACATCAGGCGTTAGTC |
a
The underlined italic sequences are the introduced restriction sites.