TABLE 3.
Results of error-prone PCR mutagenesis of fabK and selection for complementation of the ΔfabI strain FAZL1
| Isolate | FabK mutation(s) |
|---|---|
| Ran2a | A-9G |
| Ran9 | A-9G, L9 (CUU to CUC) |
| Ran16 | A-9G, D78G, L154I, I163V |
| Ran26 | A-9G, I285V |
| Ran12 | C-12G, A185V, I220V, I261V |
| Ran13a | C-12G, V84I, K124R, L148 (UUA to CUA) |
| Ran15 | C-12G, M281L |
| Ran17 | C-12G, K250 Stop (AAA to UAA) |
a
Note that in Ran2 and Ran13, two synonymous mutations occurred for residues L9 and L148, respectively. In Ran17, a nonsense mutation truncated the protein at residue K250, suggesting that the last 69 FabK residues are not essential for ENR activity.