FIG 4.

Moraxella catarrhalis requires mclS for synthesis of CL. Analysis of the PL profile of the WT, mclS insertion mutant, mclS repaired, and mclS point mutant strains of M. catarrhalis isolates O35E (A), O12E (B), and McGHS1 (C) by HP-TLC. Phospholipids were extracted by the method of Bligh and Dyer (56) and developed in one dimension using a mobile phase of chloroform-methanol-acetic acid (65:25:10). Phospholipid spots were visualized by staining with the Molybdenum Blue spray reagent. Purified CL, PG, and PE were used as standards to identify the PLs of M. catarrhalis. Arrow, location to which CL migrates upon HP-TLC analysis.