TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Selectable marker | Source |
|---|---|---|---|
| Strains | |||
| M. catarrhalis | |||
| O35E | WT isolate | None | 76 |
| O35E.mclS | mclS isogenic insertion mutant strain of O35E | Spec | This study |
| O35E.mclS repaired | Repaired strain of O35E.mclS | Stm | This study |
| O35E.mclS.K438R | mclS isogenic point mutant strain of O35E | Stm | This study |
| O35E.ZCSM | hag, uspA1, uspA2, and mcaP isogenic mutant strain of O35E | Zeo, Cat, Spec, Kan | 17 |
| O35E.SM100 | rpsL isogenic mutant strain of O35E | Stm | 77 |
| O35E.CD1 | ompcd isogenic mutant strain of O35E | Kan | 18 |
| O35E.MhaB | mhaB1 and mhaB2 isogenic mutant strain of O35E | Spec, Zeo | 19 |
| O12E | WT isolate | None | 15 |
| O12E.mclS | mclS isogenic insertion mutant strain of O12E | Spec | This study |
| O12E.mclS repaired | Repaired strain of O35E.mclS | Stm | This study |
| O12E.12Hg | hag, uspA1, and uspA2 isogenic mutant strain of O12E | Zeo, Cat, Spec | Unpublished data |
| McGHS1 | WT isolate | None | 49 |
| McGHS1.mclS | mclS isogenic insertion mutant strain of McGHS1 | Spec | This study |
| McGHS1.mclS repaired | Repaired strain of McGHS1.mclS | Stm | This study |
| McGHS1.Hag | hag isogenic mutant strain of McGHS1 | Spec | 49 |
| E. coli EPI300 | Cloning strain | None | Epicentre Illumina |
| Plasmids | |||
| pSPECr | Source of Specr cassette | Spec | 78 |
| pCC1 | Cloning vector, replicative in E. coli | Cat | Epicentre Illumina |
| pCC1.mclS | pCC1 containing mclS from O35E | Cat | This study |
| pCC1.mclS.spec | pCC1.mclS with Specr cassette inserted into the mclS ORF | Cat, Spec | This study |