TABLE 1.
Strains plasmids and cosmids used in this article
| Strain, plasmid, or cosmid | Genotype or description | Reference or source |
|---|---|---|
| Escherichia coli strains | ||
| MC1061 | F− Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ− e14− mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2(r− m+) | 52 |
| BW25113 | F− Δ(araD-araB)567 ΔlacZ4787 (::rrnB-3) λ− rpoS396(Am) rph-1 Δ(rhaD-rhaB)568 hsdR514 | 53 |
| ET12567 | dam13::Tn9 dcm6 hsdM hsdR rec143F zjj201::Tn10 galK2 galT22 ara14 lacY1 leuB1 thi1 tonA31 rpsL136 hisG4 tsx78 mtlI glnV44 F− | 54 |
| Streptomyces coelicolor strains | ||
| M145 | Wild-type derivative of S. coelicolor A3(2) lacking plasmids | 52 |
| TP4 | As M145; ΔtatC | 43 |
| APH2 | As M145; ΔqcrCAB::hyg | This work |
| APH3 | As APH2; ϕC31 (PhrdB-qcrCHis10AB) | This work |
| APH5 | As APH2; ϕC31 (PhrdB-qcrCABHis10) | This work |
| APH5-KK | As APH5 except that DNA coding for the twin arginine motif (R161 and R162) of QcrA is mutated to twin lysine codons | This work |
| APH6 | As TP4; ϕC31 (PhrdB-qcrCHis10AB) | This work |
| APH8 | As TP4; ϕC31 (PhrdB-qcrCABHis10) | This work |
| Plasmids and cosmids | ||
| pBluescript (KS+) | Cloning vector | Stratagene |
| pIJ790 | Modified λRed recombination plasmid | 35 |
| pUZ8002 | Nontransmissible oriT-mobilizing plasmid | 55 |
| pIJ10700 | pBlueScriptII (KS+) containing the hygromycin resistance gene and oriT from plasmid RP4, flanked by FLP recombination target (FRT) sites | 56 |
| Cosmid 11-D01 | SuperCos I vector containing chromosomal region 2295280 to 2330618, encoding genes sco2135 to sco2165 | Paul Dyson Swansea |
| Cosmid 11-D01(50H) | As cosmid 11-D01 except that qcrCAB (sco2150-sco2148) is deleted and replaced with FRT-oriT-hyg-FRT by homologous recombination | This work |
| pSET152 | Vector for conjugal transfer and chromosomal integration at the ϕC31 attachment site in Streptomyces | 57 |
| pAPH1 | pSET152 carrying PhrdB-qcrCHis10AB | This work |
| pAPH3 | pSET152 carrying PhrdB-qcrCABHis10 | This work |
| pAPH3KK | As APH3 except that DNA coding for the twin arginine motif of QcrA is mutated to twin lysine codons | This work |