TABLE 2.
Cytochrome c oxidase activity in solubilized membrane fractions of S. coelicolor M145 strains
| Strain | Mean cytochrome bc1 complex activity ± SDa (nmol cyt c reduced · mg−1 protein · min−1) |
|---|---|
| M145 (WT) | 217 ± 48 |
| APH2 (ΔqcrCAB::Hygr) | 1.0 ± 20 |
| TP4 (ΔtatC) | −12 ± 14 |
| APH3 (ΔqcrCAB::Hygr ϕC31 PhrdB-qcrCHis10AB) | 44 ± 8 |
| APH5 (ΔqcrCAB::Hygr ϕC31 PhrdB-qcrCABHis10) | 66 ± 20 |
| APH5-KK (ΔqcrCAB::Hygr ϕC31 PhrdB-qcrCAR161K R162KBHis10) | 4.5 ± 4.0 |
| APH6 (ΔtatC ϕC31 PhrdB-qcrCHis10AB) | −14 ± 15 |
| APH8 (ΔtatC ϕC31 PhrdB-qcrCABHis10) | 6.9 ± 7.1 |
a
Values are the means ± standard deviations of 4 replicates.