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. 2014 Jan;196(1):16–27. doi: 10.1128/JB.00930-13

FIG 2.

FIG 2

DegU∼P inhibits transcription from the epsA and tapA promoter regions. (A) Biofilm morphology indicated by the formation of a complex colony on MSgg agar after 24 h of incubation at 37°C for the wild-type strain NCIB3610 (3610) and strain degU P(Hy)-spank-degU32(Hy)-lacI (NRS1325); 25 μM IPTG was used to induce transcription in strain NRS1325, as indicated. (B) Schematic representation of the role of DegU∼P and Spo0A∼P in controlling biofilm formation by B. subtilis. The broken red box highlights our hypothesis that high levels of DegU∼P inhibit transcription from the matrix operons. (C) Schematic representation of the degU32(Hy) strain (NRS1325) used in the analysis. (D) β-Galactosidase activity generated from the PbslA-lacZ reporter fusion in wild-type strain 3610 (NRS2052) and the degU32(Hy) strain [degU P(Hy)-spank-degU32(Hy)-lacI (NRS2226)]. (E) As for panel D, but data are shown for the PepsA-lacZ reporter fusion in the wild-type strain 3610 (NRS1529) and the degU32(Hy) strain [degU P(Hy)-spank-degU32(Hy)-lacI (NRS1553)]. (F) As for panel D, but data are shown for the PtapA-lacZ reporter fusion in wild-type strain 3610 (NRS1503) and the degU32(Hy) strain [degU P(Hy)-spank-degU32(Hy)-lacI (NRS1515)]. Data presented in panels D to F are averages from 3 experiments, where the error bars represent the standard error of the mean. IPTG was added as indicated.