TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and/or descriptionc | Source or reference |
|---|---|---|
| Bacillus subtilis strains | ||
| 168 | Wild type; trpC2 | Laboratory stock |
| PS832 | Wild-type trpC2 revertant of strain 168 | Laboratory stock |
| PERM454a | ΔexoA::tet Δnfo::neo Tetr Neor | 15 |
| PERM733a | ΔdisA::lacZ Eryr | pPERM732→PS832b |
| PERM758a | ΔexoA::tet Δnfo::neo ΔdisA Tetr Neor Eryr | pPERM732→PERM454b |
| PERM850a | ΔexoA::tet Δnfo::neo ΔdisA amyE::PsspB-disA Tetr Neor Eryr Cmr | pPERM842→PERM758b |
| PERM1008a | disA-gfp Eryr | pPERM1007→PS832b |
| PERM1197a | amyE::PsspB-disA Cmr | pPERM842→PS832b |
| Plasmids | ||
| pMUTIN4 | Integrational B. subtilis vector; Ampr Eryr | 16 |
| pMUTIN-GFP | Integrational B. subtilis vector; Ampr Eryr | 17 |
| pDG364 | Integrational B. subtilis vector (integrates into the amyE locus of B. subtilis); Ampr Cmr | Wayne Nicholson |
| pPERM615 | 674-bp fragment containing the B. subtilis sspB promoter cloned between the EcoRI-BamHI sites of pDG364; Ampr Cmr | 18 |
| pPERM732 | 307-bp internal fragment of the disA ORF inserted between the EcoRI-BamHI sites of pMUTIN4; Ampr Eryr | This study |
| pPERM842 | 1,293-bp DNA fragment of the B. subtilis disA ORF cloned into the BamHI site of pPERM615, generating a PsspB-disA construct; Ampr Cmr | This study |
| pPERM1007 | 1,108-bp DNA fragment encompassing 28 bp upstream from the start codon to the last codon of the disA ORF cloned between the KpnI-ClaI sites of pMUTIN-GFP; Ampr Eryr | This study |
a
The background for this strain is PS832.
b
DNA of the plasmid to the left of the arrow was used to transform the strain to the right of the arrow.
c
Amp, ampicillin; Cm, chloramphenicol; Ery, erythromycin; Neo, neomycin; Tet, tetracycline.