TABLE 2.

Quantification of EndoIV and Fpg degradation of chromosomal DNA isolated from dormant or outgrowing spores of different B. subtilis strains^a

Enzyme	Avg % band intensity ± SD remaining after enzyme treatment of strainb:
	WT		nfo exoA		nfo exoA disA
	DS	OG	DS	OG	DS	OG
EndoIV	87 ± 9.8	94 ± 8.8	86 ± 7.6	75 ± 8.6	47 ± 5.1	12 ± 0.7
Fpg	18 ± 2.1	97 ± 9.2	9 ± 1.1	67 ± 7.3	10.5 ± 1.2	8.5 ± 0.94

^aSamples of chromosomal DNA isolated from outgrowing spores (OG) or dormant spores (DS) of each strain were treated with EndoIV or Fpg, respectively, and reaction mixes were electrophoresed on a 1% alkaline agarose gel and stained with ethidium bromide, all as described in Materials and Methods and the legend to Fig. 1A to C.

^bFor each reaction, the intensity of the chromosomal DNA band remaining in the gel well after EndoIV or Fpg treatment was quantified by densitometry using Quantity One 1-D software (Bio-Rad Laboratories, Hercules, CA), using as a reference the chromosomal DNA band in the gel well from an identical amount of total chromosomal DNA in an untreated control reaction that was run in an adjacent lane to the same amount of total EndoIV or Fpg-treated chromosomal DNA, as shown in Fig. 1A to C. Values are averages ± standard deviations for duplicate determinations in two separate experiments (with different lots of spores).