TABLE 3.
Time of analysis after germination onset (min) | No. of cell chains analyzed | No. of cells/chain | No. of septa/chain | No. of chains with 1 DisA fluorescent focus | No. of chains with 2 DisA fluorescent foci |
---|---|---|---|---|---|
120 | 200 | 2–4 | 1–3 | 184 | 16 |
180 | 200 | 4–8 | 3–7 | 186 | 14 |
Spores of strain PERM1008 (disA-gfp) were germinated and outgrown in 2× SG medium at 37°C, and at various times, cells were examined by microscopy as described in Materials and Methods.