TABLE 2.

Primers for pMNN1-phoZ reporter constructs

Plasmid construct	Primer name	Sequence
pMNN1-salA-pro	5′-salA-pro-BamHI	CGC GGA TCC GAA TGA TTT TGA GAT ACT TC
	3′-salA-pro-EcoRI	CCG GAA TTC CTT CTT CGA TAG CAT TAG TC
pMNN1-salK-pro	5′-salK-pro-BamHI	CGC GGA TCC CAT TGC GGT CAG TGA CAT CC
	3′-salK-pro-EcoRI	CCG GAA TTC GCC AGC CAA ATA AAA GCT G
pMNN1-salKR-pro	5′-salY-BamHI	CGC GGA TCC GAT AGA GGA GAG TAA TAT G
	3′-salY-EcoRI	CCG GAA TTC CAT ATT ATT TAC TTA ATC G
pMNN1-salKR-pro-del1	5′-salK-pro-del1	CGC GGA TCC GTT ATC GCT AAT GGG TCA TTG
	3′-salY-EcoRI	CCG GAA TTC CAT ATT ATT TAC TTA ATC G
pMNN1-salKR-pro-del2	5′-salK-pro-del2	CGC GGA TCC GAC TCC ATC TGC CAT AG
	3′-salY-EcoRI	CCG GAA TTC CAT ATT ATT TAC TTA ATC G
pMNN1-salKR-pro-del3	5′-salK-pro-del3	CGC GGA TCC CAG TTT AGT ATG GTT CCT G
	3′-salY-EcoRI	CCG GAA TTC CAT ATT ATT TAC TTA ATC G
pMNN1-salKR-pro-short	5′-salK-pro-del2	CGC GGA TCC GAC TCC ATC TGC CAT AG
	3′-salY-pro-short-EcoRI	CCG GAA TTC GTT TTT CCA AGG CTA ATT TCT G
pMNN1-salKR-pro-short-flip	5′-salKR-pro-del2-EcoRI	CCG GAA TTC GAC TCC ATC TGC CAT AG
	3′-salK-pro-short-BamHI	CGC GGA TCC GTT TTT CCA AGG CTA ATT TCT G
pMNN1-salKR-pro-del2A	5′-salYK-pro-del2A	CGC GGA TCC GAG TTA ATG AAA GCT CAA GG
	3′-salY-EcoRI	CCG GAA TTC CAT ATT ATT TAC TTA ATC G