TABLE 1.
Genotypea | Biochemical activity removed by mutation | Lac |
---|---|---|
Wild type | None | + |
ΔtruA | tRNA pseudouridine synthase, anticodon stem-loop specific | + |
ΔtruB | tRNA pseudouridine synthase, Ψ55 specific | + |
ΔrluA | Pseudouridine synthase, 23S rRNA and tRNAphe specific | + |
ΔrluC | Pseudouridine synthase, 23S rRNA, positions 955, 2504, and 2580 | + |
ΔrsuA | Pseudouridine synthase, 16S rRNA position 516 | + |
ΔmiaA | UXX codon tRNA prenyl transferase at position 37 | – |
ΔmiaB | UXX codon tRNA methylthiolase following prenylation | + |
The mutations were transduced into the wild-type rpoS750-lacZ translational fusion strain (EM1050) and tested for expression phenotypes on MacConkey-lactose plates incubated overnight at 37°C. Strain names are given in Table S1 in the supplemental material.